Agilent 300SB-C18, 4.6x100mm 3.5um 861973-902

The Agilent ZORBAX 300SB-C18 (catalog number 861973-902) is a reversed‑phase HPLC column (4.6 × 100 mm, 3.5 µm) built on a 300 Å wide‑pore StableBond silica phase for the reproducible separation of proteins, peptides and other large biomolecules. It is intended for LC and LC‑MS workflows that require good recovery of high‑molecular‑weight analytes and enhanced chemical stability at low pH (suitable for TFA, formic or acetic acid modifiers).

Key Features:

• Separation mode: Reversed‑phase (RP).
• Chemistry/Phase: ZORBAX 300SB‑C18 (StableBond C18).
• Particle size: 3.5 µm (fully porous).
• Pore size: 300 Å (wide pore for proteins/peptides).
• Dimensions: 4.6 mm ID × 100 mm length.
• Recommended pH range: ~1–8 (stable at low pH).

Advantages:

• Wide 300 Å pores allow efficient access and separation of proteins and large peptides, improving recovery and peak shape for high‑molecular‑weight analytes.
• StableBond chemistry provides enhanced stability in low‑pH mobile phases (useful with TFA or for LC/MS at low pH), reducing silica dissolution and extending column life.
• 3.5 µm particle size offers a balance of efficiency and backpressure for robust HPLC and LC/MS methods on conventional instrumentation.