Agilent 300SB-C18 Analytical HPLC Col 4.6 x 250 880995-902

The Agilent ZORBAX 300SB-C18 (part number 880995-902) is an analytical reversed-phase HPLC column (4.6 x 250 mm) packed with 5 µm, 300 Å fully porous StableBond C18 packing. It is designed for reliable separation and analysis of larger biomolecules such as peptides and proteins, and is intended for LC and LC–MS applications where low-pH mobile phases (e.g., TFA-containing solvents) are used. The column is offered for biopharma workflows that require wide-pore media and StableBond chemistry to improve recovery and reproducibility.

Key Features:

• Phase: ZORBAX 300SB-C18 (StableBond C18)
• Dimensions: 4.6 mm ID × 250 mm length
• Particle size: 5 µm
• Pore size: 300 Å
• pH range: 1–8
• USP classification: L1
• Separation mode: Reversed‑phase

Advantages:

• StableBond chemistry provides enhanced stability in low‑pH mobile phases (suitable for TFA, formic or acetic acid modifiers), reducing degradation in acidic conditions.
• 300 Å wide pores and 5 µm particles improve access and recovery for peptides, proteins and other large molecules compared with standard pore columns.
• Rated for HPLC operating conditions (high pressure and elevated temperature capability), enabling robust, reproducible runs and improved efficiency for difficult proteins when operated at higher temperatures.