Agilent 300SB-C3 Analytical HPLC Column 4.6x250 880995-909
The Agilent ZORBAX 300SB-C3 analytical HPLC column (part number 880995-909) is a reversed-phase silica column with a C3 StableBond bonded phase designed for separation and analysis of larger biomolecules. It uses wide 300 Å pores and a 5 µm fully porous particle to allow proteins and peptides to enter the stationary phase, and is specified for LC and LC/MS peptide/protein applications with low-pH mobile phases.
Key Features:
- Dimensions: 4.6 × 250 mm (ID × length).
- Particle size: 5 µm (fully porous).
- Pore size: 300 Å.
- Phase/Chemistry: 300SB-C3 (StableBond C3).
- Separation mode: Reversed‑phase (RP) for peptides/proteins.
- pH range: 1–8; maximum operating temperature up to 80 °C.
- USP designation L56; pressure rating ~400 bar.
Advantages:
- 300 Å pore size improves recovery and resolution of proteins and larger peptides by allowing efficient access to the bonded phase.
- StableBond C3 chemistry provides enhanced stability at low pH (compatible with TFA, formic or acetic acid), supporting LC/MS workflows.
- High temperature tolerance (up to 80 °C) and 400 bar pressure rating increase method robustness and help improve separation efficiency for difficult protein/peptide analyses.
Product Details
Overview
| Model | |
|---|---|
| Product Type | Consumables |
| Part Number | 880995-909 |
| Brand | Agilent Technologies |
| Gross Dimensions (WxDxH cm) | |
| Net Dimensions (WxDxH cm) | |
| Gross Weight (kg) | |
| Net Weight (kg) |
Specifications
| Separation Mode | Reversed Phase |
|---|---|
| Particle Size | 5 µm |
| Length | 250 mm |
| Inner Diameter | 4.6 mm |
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