Agilent 300SCX A Analytical HPLC Column 4.6x250 880952-714

The Agilent ZORBAX 300SCX A (part number 880952-714) is a strong cation‑exchange analytical HPLC column (4.6 x 250 mm) packed with 5 µm spherical bonded silica. It is designed for efficient separations of basic, water‑soluble compounds and biomolecules (including peptides and proteins) and the “A” version is modified for reduced retention to enable faster methods. Typical uses include ion‑exchange separations, method development for small basic analytes, and biomolecule purification/analysis.

Key Features:

• Separation Mode: Strong cation exchange (Ion Exchange, SCX)
• Chemistry: ZORBAX bonded sulfonic‑acid phase on silica
• Particle Size: 5 µm (spherical bonded silica)
• Length: 250 mm
• Inner Diameter: 4.6 mm
• Pore Size: 300 Å
• pH Range: 2–7 (operational)

Advantages:

• High efficiency for basic and water‑soluble analytes and biomolecules, suitable for proteins and peptides.
• 300 Å pores and 5 µm particles provide compatibility with larger biomolecules while maintaining good chromatographic efficiency.
• Robust operational window (pH 2–7 and high mechanical stability) supports higher flow rates and pressures for faster runs.
• “A” low‑retention modification enables shorter run times and faster re‑equilibration for rapid method workflows.