Agilent Extend-C18 Analytical, 5um, 4.6x150mm 773450-902

The Agilent ZORBAX Extend-C18 Analytical column (part number 773450-902) is a reversed-phase HPLC analytical column (C18 chemistry) sized 4.6 x 150 mm with 5 µm particles. It is designed for high-pH separations and routine LC/MS and biopharma work, offering improved retention, peak shape and resolution for basic compounds, peptides and proteins. The column chemistry and manufacturing steps provide stability and reproducible performance across a wide pH range for method flexibility and improved sensitivity in LC/MS workflows.

Key Features:

• Phase/chemistry: ZORBAX Extend-C18 (C18, Extend-C18)
• Dimensions: 4.6 mm inner diameter × 150 mm length
• Particle size: 5 µm (fully porous, spherical)
• Pore size: 80 Å
• pH range: 2.0–11.5
• USP classification: L1
• Pressure rating: up to 400 bar

Advantages:

• High-pH stability (up to pH 11.5) enables better retention and peak shape for basic analytes that perform poorly at low pH.
• Improved LC/MS sensitivity for peptides and small molecules compared with some TFA-containing mobile phases.
• Robust Extend-C18 chemistry (bidentate silane and double endcapping) resists silica dissolution and extends column lifetime at elevated pH.
• Versatile reversed‑phase performance suitable for biopharma and general analytical HPLC separations.