Agilent Extend-C18,4.6x50mm,1.8um,600bar 727975-902

The Agilent ZORBAX Extend-C18 (part number 727975-902) is a reversed‑phase UHPLC analytical column (4.6 x 50 mm, 1.8 μm) designed for high‑pH separations and routine UHPLC work. It uses Agilent's Extend‑C18 chemistry with a bidentate (two‑tooth) bonding approach and double endcapping that protects silica and enables stable operation across a wide pH range (reported to pH 11.5). Typical use cases include improved separations and peak shape for basic compounds, high‑pH method development, and LC–MS analysis of peptides and small molecules where sensitivity benefits from high‑pH modifiers such as ammonium hydroxide.

Key Features:

• Chemistry: ZORBAX Extend‑C18 (C18, double endcapped)
• Dimensions: 4.6 mm ID × 50 mm length
• Particle size: 1.8 μm (fully porous)
• Pore size: 80 Å
• Pressure rating: 600 bar (RRHT 1.8 μm columns)
• pH stability range: pH 2.0 to 11.5
• USP classification: L1 (C18)

Advantages:

• High pH stability (to pH 11.5) from bidentate bonding and double endcapping for extended column lifetime
• Improved retention, resolution and peak shape for basic analytes compared with conventional silica phases
• Enhanced LC–MS sensitivity for peptides and small molecules when using high‑pH mobile phase modifiers (e.g., ammonium hydroxide)
• UHPLC performance at high pressure (1.8 μm particles rated to ~600 bar) for faster, higher‑efficiency separations