Agilent HC-C18, 4.6x250mm, 5u 518905-902

The Agilent HC-C18 (part number 518905-902) is an analytical reversed-phase HPLC column packed with a C18 (octadecyl) stationary phase in 5 µm fully porous particles, sized 4.6 x 250 mm for routine and method-development separations. It is intended for high-resolution separations of small to moderately polar organic molecules on conventional HPLC systems and is supplied with endcapped chemistry to improve peak shape. Manufacturer specifications list a pH range of 2–9, a maximum temperature of 60 °C and a pressure rating of 400 bar.

Key Features:

• Phase / Chemistry: C18 (octadecyl).
• Particle size: 5 µm (fully porous).
• Dimensions: 4.6 mm ID × 250 mm length.
• Separation mode: Reversed‑phase HPLC.
• USP classification: L1 (C18).
• Operating limits: pH range 2–9; max temperature 60 °C; pressure rating 400 bar.

Advantages:

• Designed for robust reversed-phase separations of small molecules, making it suitable for routine HPLC analyses and method development as stated by the manufacturer.
• Endcapped C18 chemistry reduces residual silanol interactions and improves peak shape and reproducibility for basic and polar analytes (inferred from manufacturer’s 'endcapped' specification).
• Wide pH tolerance (2–9) and 400 bar pressure rating provide operational flexibility across common mobile phases and allow use on conventional HPLC instruments under varied conditions.
• USP L1 designation indicates compatibility with standard C18 methods, facilitating method transfer and comparability with pharmacopeial procedures.