Agilent Microsorb-MV 300-5 C4 250 x 4.6mm R0086503C5

Microsorb-MV 300-5 C4 250 x 4.6 mm is an Agilent HPLC reversed-phase column packed with butylsilane-bonded silica (C4) having 300 Å pores and 5 µm particles. The packing is fully end-capped with trimethylsilyl groups to minimize silanol activity. The column is designed for reversed-phase separations of relatively hydrophobic peptides and proteins and for chromatographic scale-up and separation of larger proteins.

Key Features:

• Phase: C4 (butylsilane-bonded silica)
• Particle size: 5 µm
• Pore size: 300 Å
• Column dimensions: 250 x 4.6 mm
• Endcapped: Fully end-capped with trimethylsilyl groups
• Product family: Microsorb-MV (columns tested in batches rather than individually)

Advantages:

• Optimized retention and separation of relatively hydrophobic peptides and proteins that are too strongly retained on C8/C18 phases.
• 300 Å pore size and 5 µm particles support separation of larger proteins and chromatographic scale-up.
• Full endcapping reduces secondary silanol interactions, improving peak shape for basic or polar analytes.
• MV batch-tested production offers lower-cost columns while maintaining the same packing material and performance as regular Microsorb columns.