Agilent Zorbax Extend C18, 3.5um, 4.6 x 100mm 764953-902

ZORBAX Extend-C18 is a reversed-phase HPLC column phase offered by Agilent Technologies designed for high-efficiency separations, particularly for basic compounds using high-pH mobile phases. Official product descriptions state the Extend-C18 chemistry uses a bidentate C18-C18 bonding approach with double endcapping to provide stability of silica-based columns at elevated pH, enabling separations up to pH 11.5 and supporting LC and LC/MS workflows. Typical use cases given by the manufacturer and authorized distributors include high-pH reversed-phase analyses to improve peak shape, retention, and resolution of basic analytes and to enable sensitive LC/MS separations with volatile basic buffers.

Key Features:

• Column dimensions: 4.6 mm inner diameter x 100 mm length
• Particle size: 3.5 µm (fully porous)
• Phase: ZORBAX Extend-C18 (double endcapped, bidentate C18-C18 bonding)
• Pore size: 80 Å
• pH stability range: pH 2 to 11.5
• Pressure rating: 400 bar
• Carbon load: 12.5%

Advantages:

• High-pH stability (stated pH range to 11.5) for robust separations of basic compounds.
• Improved peak shape and resolution for basic analytes due to bidentate bonding and double endcapping as described by the manufacturer.
• High efficiency suitable for HPLC methods with 3.5 µm particles and a 400 bar pressure rating.
• Enhanced LC/MS sensitivity when using volatile basic mobile phases (manufacturer/authorized-distributor guidance cites ammonium hydroxide at pH ~10.5).