Hygiena foodproof Brucella Detection Kit, 5'Nuclease KIT230040

The foodproof Brucella Detection Kit is a real-time PCR assay for rapid detection of Brucella spp. and discrimination of Brucella abortus and Brucella melitensis in food and related samples. The kit uses 5' Nuclease hydrolysis probe chemistry and includes an internal amplification control and contamination prevention enzymes to deliver reliable qualitative results for food safety testing and monitoring. The product is identified as KIT230040 and is intended for use on compatible real-time PCR instruments with FAM, VIC/HEX, ROX/Texas Red and Cy5 detection channels.

Key Features:

• Reactions: 96 (designed for up to 94 samples plus controls; 25 µL final reaction volume)
• Targets: Brucella spp., Brucella abortus, Brucella melitensis
• Chemistry: 5' Nuclease hydrolysis probe assay (multiplexed)
• Controls & contamination prevention: Internal amplification control included; heat-labile Uracil-DNA Glycosylase (UNG) provided
• Instrument channels: FAM, VIC/HEX, ROX/Texas Red and Cy5 compatible
• Storage: kit components stored at -15°C to -25°C

Advantages:

• Rapid, specific detection and species discrimination of Brucella directly from enriched food samples
• Built-in internal control and UNG-based carryover prevention improve result reliability and reduce false positives
• Ready-to-use master mix and probe formulation minimize hands-on pipetting and simplify assay setup
• Multiplex design allows simultaneous detection of genus and two key species in a single reaction